Bacterial Genetics and Genomics, Chapter 8, Discussion topic 2. As described in Chapter 8 of Bacterial Genetics and Genomics, a two-component regulatory system has a sensory protein in the membrane that autophorphorylates when triggered by its sensory signal and a regulatory protein that is phosphorylated to do its job adjusting gene expression. In this way, somethingContinue reading “What does a two-component regulator do when it isn’t phosphorylated?”
Tag Archives: DNA
A single amino acid change can have a big impact on the bacteria.
Bacterial Genetics and Genomics, Chapter 7, Discussion topic 2. It doesn’t take much to alter the sequence of a protein. Whilst some single nucleotide changes to the DNA sequence can be tolerated, others cause the gene to encode a different amino acid in the protein. Again, some of these changes to the amino acid sequence canContinue reading “A single amino acid change can have a big impact on the bacteria.”
Harnessing aptamers for rapid detection of bacteria and antibiotics in our food.
Bacterial Genetics and Genomics book Discussion Topic: Chapter 4, question 14 The specificity of antibody binding has been exploited for many years in a variety of technologies. Although perhaps less famous, aptamers also have high binding specificity for their targets and, being made of RNA or DNA rather than protein, are much smaller in size,Continue reading “Harnessing aptamers for rapid detection of bacteria and antibiotics in our food.”
Biology Week Topic: Genomics, Earthquakes, and Cholera
Bacterial Genetics and Genomics book Discussion Topic: Chapter 11, question 14 Although I had planned to continue to work through the Discussion Topics from Bacterial Genetics and Genomics that are all related to the investigations in bacterial genetics and genomics using bioinformatics tools can be conducted outside of the lab, October here in the UKContinue reading “Biology Week Topic: Genomics, Earthquakes, and Cholera”
Bacterial genomes, then and now
Bacterial Genetics and Genomics book Discussion Topic: Chapter 17, question 13 The publications of the first bacterial genome sequences were 25 years ago. The technology has come a long way since then, both in the lab and computationally. One of the first bacterial genome sequencing projects started was one undertaken to sequence the complete EscherichiaContinue reading “Bacterial genomes, then and now”
Research and planning outside of the lab: working with restriction enzymes.
Bacterial Genetics and Genomics book Discussion Topic: Chapter 16, question 15 For this blog, I have decided to look at the Discussion Topic from Bacterial Genetics and Genomics, Chapter 16, question 15, which discusses restriction enzymes and encourages us to try finding digest sites for these enzymes ourselves in a gene of interest, using on-lineContinue reading “Research and planning outside of the lab: working with restriction enzymes.”
Bacterial Genetics and Genomics, by Lori A. S. Snyder 